Journal of Oral and Maxillofacial Pathology

: 2020  |  Volume : 24  |  Issue : 2  |  Page : 204--205

“Single file” pattern in histopathology

Deepak Pandiar1, T Smitha2,  
1 Department of Oral and Maxillofacial Pathology, Postgraduate Institute of Dental Sciences, Rohtak, Haryana, India
2 Department of Oral and Maxillofacial Pathology, V S Dental College and Hospital, Bengaluru, Karnataka, India

Correspondence Address:
T Smitha
Department of Oral and Maxillofacial Pathology, V S Dental College and Hospital, Bengaluru, Karnataka

How to cite this article:
Pandiar D, Smitha T. “Single file” pattern in histopathology.J Oral Maxillofac Pathol 2020;24:204-205

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Pandiar D, Smitha T. “Single file” pattern in histopathology. J Oral Maxillofac Pathol [serial online] 2020 [cited 2021 Jan 21 ];24:204-205
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“Single file” arrangement of the tumor cells is a feature of various malignancies, and the cells are seen arranged in linear rows which appear as if one following the footsteps of another.[1] The term has been used interchangeably with “Indian file” probably denoting Amerindians people's way of walking along a trail as illustrated in [Figure 1]a. The pattern may be appreciated both cytologically and histologically. Sometimes, this pattern is also referred to as “cord-like” pattern as characterized by the presence of single line of tumor cells; this is considered to be highly characteristic of lobular carcinoma of the breast [Figure 1]b.{Figure 1}

Although, lobular carcinoma is a distinctive feature of breast; in head-and-neck region, this pattern has also been described commonly in polymorphous adenocarcinoma, and malignant epithelial tumor characterized by cytological uniformity, morphological diversity and an infiltrative growth pattern.[2] On case-by-case basis, the term “low grade” may be added. Morphological diversity is seen as the tumor cells grow in solid pattern, cribriform pattern, large cystic space, or cord-like. As aforementioned, the tumor shows infiltrative growth pattern particularly at the periphery. This area shows tumor cells proliferating in single file fashion that resembles “beads on a string” [Figure 1]c.[3] A corresponding hand drawn picture of single file pattern in polymorphous adenocarcinoma is illustrated in [Figure 1]d. It was demonstrated that polymorphous low-grade adenocarcinoma contained a significantly higher proportion of reticular, tubular and single file patterns as compared to cribriform adenocarcinoma of the salivary gland containing a higher percentage of papillary, solid and cribriform patterns.[4] This distinction has been omitted from the recent WHO Classification of Head and Neck Tumors.[2] When cribriform pattern predominates, the distinction from adenoid cystic carcinoma may be difficult; howbeit, polymorphous adenocarcinoma shows cytological uniformity and consistent p63+/p40− profile.[5]

Sclerosing odontogenic carcinoma is another rare odontogenic malignancy, which histologically demonstrates single file cord-like infiltrative odontogenic epithelium in sclerosing stroma,[2] albeit the phenomenon is not pathognomonic. It is not unusual to find similar pattern of invasion in oral squamous cell carcinoma, particularly at the invasive tumor front [Figure 2].{Figure 2}

Interchangeably, the term is used with cord-like pattern as mentioned earlier. Vulval angiomyofibroblastoma shows small cord-like myofibroblastic cells. Rare examples of myxoid liposacrcomas feature cell rows which are separated either by myxoid or hyalinized stroma. A similar cord-like pattern is also seen in some cases of hydropic and vascular leimyomas.[6]

Rajesh et al. and Hemachandran and Dey in independent cytological studies demonstrated Indian file pattern in various adenocarcinomas; the type of specimen studied however was different.[7],[8] Latter authors demonstrated the pattern in 7 out of 25 effusion specimens and suggested that when adenocarcinoma cells infiltrate the tissue, they oftentimes evoke a desmoplastic reaction. Further, these carcinoma cells then become entrapped in a linear Indian file configuration.[8]

The characteristic pattern of growth in classic invasive lobular carcinoma involves the infiltration of single file of the cells through the stroma with little accompanying disturbance of the normal architecture of the tissue.[9] Thus, in connotation with Amerindians way of walking along the trail, which was probably an art of camouflaging for survival, it may be prudent to believe that single file arrangement of the neoplastic cells may be an attempt to infiltrate the tissue without being caught by the body's immune reaction, one of the “hallmarks of cancer.”.

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1Rekhtman N, Baine MK, Bishop JA. Quick Reference Handbook for Surgical Pathologists. 2nd ed. New York: Springer Nature Switzerland AG; 2019. p. 168.
2El-Naggar AK, Chan JK, Grandis JR, Takata T, Slootweg PJ, editors. Odontogenic and maxillofacial tumors. In: WHO Classification of Tumours of the Head and Neck. 4th ed. Lyon: IARC Press; 2017. p. 203-60.
3Paleri V, Robinson M, Bradley P. Polymorphous low-grade adenocarcinoma of the head and neck. Curr Opin Otolaryngol Head Neck Surg 2008;16:163-9.
4Xu B, Aneja A, Ghossein R, Katabi N. Predictors of outcome in the phenotypic spectrum of polymorphous low-grade adenocarcinoma (PLGA) and cribriform adenocarcinoma of salivary gland (CASG): A retrospective study of 69 patients. Am J Surg Pathol 2016;40:1526-37.
5Rooper L, Sharma R, Bishop JA. Polymorphous low grade adenocarcinoma has a consistent p63+/p40- immunophenotype that helps distinguish it from adenoid cystic carcinoma and cellular pleomorphic adenoma. Head Neck Pathol 2015;9:79-84.
6Al-Nafussi A. Tumour Diagnosis: Practical Approach and Pattern Analysis. 2nd ed. London: Hodder Arnold; 2005. p. 23.
7Rajesh L, Dey P, Joshi K. Fine needle aspiration cytology of lobular breast carcinoma. Comparison with other breast lesions. Acta Cytol 2003;47:177-82.
8Hemachandran M, Dey P. Indian file pattern of adenocarcinoma cells in effusions. Acta Cytol 2004;48:385-6.
9McCart Reed AE, Kutasovic JR, Lakhani SR, Simpson PT. Invasive lobular carcinoma of the breast: Morphology, biomarkers and 'omics. Breast Cancer Res 2015;17:12.