Year : 2018 | Volume
: 22 | Issue : 3 | Page : 353--359
Low frequency of BRAF V600E immunoexpression in mandibular ameloblastomas: An institutional study
Pankaj M Shirsat, Shivani Bansal, Pooja Prasad, Rajiv S Desai
Department of Oral Pathology, Nair Hospital Dental College, Mumbai, Maharashtra, India
Background: Ameloblastoma is an uncommon, benign neoplasm of odontogenic epithelium commonly affecting the posterior mandible (almost 80%) particularly in the molar/ramus region, with occasional tumors involving the maxilla. Recently, there has been much interest generated after the reports of BRAF V600E mutations in ameloblastomas with a frequency of 46%–80% using both molecular as well as immunohistochemical (IHC) techniques. We sought to assess the presence of BRAF V600E expression in ameloblastomas in Indian patients and correlate the same with clinical behavior and histological variants by performing IHC analysis with anti-BRAF V600E antibody.
Materials and Methods: Thirty formalin-fixed paraffin-embedded tissues of mandibular ameloblastomas were examined by anti-BRAF V600E antibody and correlated with clinicopathologic and histological parameters. Cytoplasmic staining of neoplastic epithelium was considered positive for BRAF V600E expression.
Statistical Analysis: Data analysis was performed using Chi-square test and Student's t-test with statistical software IBM SPSS statistics 20.0.
Results: BRAF V600E antibody showed positive expression only in 33.3% (10/30) cases. About 66.7% (4/6) recurrent cases and 63.6% (7/11) plexiform cases showed statistically significant association of P = 0.05 and P = 0.021, respectively, among cases with positive BRAF V600E immunoexpression.
Conclusion: We report the lowest frequency (33.3%) of BRAF V600E immunoexpression in mandibular ameloblastomas in Indian population. However, a significant association of BRAF V600E-positive immunoexpression with recurrence and plexiform pattern could underline its role as a therapeutic marker for ameloblastoma.
Rajiv S Desai
Department of Oral Pathology, Nair Hospital Dental College, Dr. A. L. Nair Road, Mumbai Central, Mumbai - 400 008, Maharashtra
|How to cite this article:|
Shirsat PM, Bansal S, Prasad P, Desai RS. Low frequency of BRAF V600E immunoexpression in mandibular ameloblastomas: An institutional study.J Oral Maxillofac Pathol 2018;22:353-359
|How to cite this URL:|
Shirsat PM, Bansal S, Prasad P, Desai RS. Low frequency of BRAF V600E immunoexpression in mandibular ameloblastomas: An institutional study. J Oral Maxillofac Pathol [serial online] 2018 [cited 2022 Jan 20 ];22:353-359
Available from: https://www.jomfp.in/article.asp?issn=0973-029X;year=2018;volume=22;issue=3;spage=353;epage=359;aulast=Shirsat;type=0