Journal of Oral and Maxillofacial Pathology

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Year
: 2017  |  Volume : 21  |  Issue : 2  |  Page : 299--300

Ultrastructural changes in cell death


BK Charan Gowda1, Ganganna Kokila2,  
1 Dental Care and Research Center, Bengaluru, Karnataka, India
2 Department of Oral Pathology, Sri Siddhartha Dental College, Sri Siddhartha Academy of Higher Education, Tumakuru, Karnataka, India

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How to cite this article:
Charan Gowda B K, Kokila G. Ultrastructural changes in cell death.J Oral Maxillofac Pathol 2017;21:299-300


How to cite this URL:
Charan Gowda B K, Kokila G. Ultrastructural changes in cell death. J Oral Maxillofac Pathol [serial online] 2017 [cited 2022 Sep 30 ];21:299-300
Available from: https://www.jomfp.in/text.asp?2017/21/2/299/213184


Full Text

Necrotic cell death or necrosis is characterized morphologically by gain in cell volume, swelling of organelles, plasma membrane rupture and subsequent loss of intracellular contents.[1],[2]

Although various alterations in organelles and cellular processes are considered in necrotic cell death, exact correlation between them is not yet drawn. Some of the changes considered include as follows.[2],[3],[4]

Mitochondrial alterations – uncoupling, production of reactive oxygen species (ROS), nitroxidative stress by nitric oxide or similar compounds and mitochondrial membrane permeabilization often controlled by cyclophilin DLysosomal changes – ROS production by Fenton reactions, lysosomal membrane permeabilizationNuclear changes – hyperactivation of poly (ADP-ribose) polymerase-1 and concomitant hydrolysis of NAD+Lipid degradation following activation of phospholipases, lipoxygenases and sphingomyelinasesIncrease in the cytosolic concentration of calcium that results in mitochondrial overload and activation of noncaspase proteases calpains and cathepsins.

Following cell death, various morphologic changes occur within cell which can be initially noted at microscopic level, which later becomes manifested as gross changes.[5] With the help of transmission electron microscopy, the intracellular changes in the oral epithelial cell following cell death in postmortem tissues were demonstrated.

At 0–2 h – The cell was intact with no changes in cytoplasmic membrane and cellular organelles [Figure 1]a,[Figure 1]b,[Figure 1]c{Figure 1}At 12th h – The epithelial cell showed nuclear, cytoplasmic and mitochondrial vacuolation, while the cell junction was found to be intact [Figure 2]a and [Figure 2]b{Figure 2}At 24th h – The cell showed increased cytoplasmic vacuolation, intact nucleus and intercellular junction, while there was enlargement of mitochondria and endoplasmic reticulum [Figure 3]a and [Figure 3]b{Figure 3}At 36th h – Intracellular organelles were not discernible [Figure 4].{Figure 4}

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Conflicts of interest

There are no conflicts of interest.

References

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2Kroemer G, Galluzzi L, Vandenabeele P, Abrams J, Alnemri ES, Baehrecke EH, et al. Classification of cell death: Recommendations of the Nomenclature Committee on Cell Death 2009. Cell Death Differ 2009;16:3-11.
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4Nicotera P, Melino G. Regulation of the apoptosis-necrosis switch. Oncogene 2004;23:2757-65.
5Kumar V, Abbas AK, Fausto N, Robbins and Cotran Pathologic basis of Disease. 7th edition, New Delhi: Elsevier, 2004. p. 3-46.