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An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
ORIGINAL ARTICLE Table of Contents   
Year : 2017  |  Volume : 21  |  Issue : 1  |  Page : 36-40
Redefining epithelial characterization in oral submucous fibrosis using morphometric analysis


1 Department of Oral and Maxillofacial Pathology and Microbiology, ITS Centre for Dental Studies and Research, Ghaziabad, Uttar Pradesh, India
2 ITS Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India
3 Department of Oral and Maxillofacial Pathology, SDM College of Dental Sciences and Hospital, Dharwad, Karnataka, India

Correspondence Address:
Amit Gupta
Department of Oral and Maxillofacial Pathology and Microbiology, I.T.S Dental College, Hospital & Research Centre, Greater Noida, Uttar Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-029X.203792

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Background: Oral submucous fibrosis (OSMF) is a chronic oral mucosal disease characterized by progressive deposition of collagen in subepithelial connective tissue and epithelial atrophy. The present study was conducted to evaluate the changes in epithelial thickness and cellular and nuclear morphometric characteristics of parabasal and spinous compartments of the epithelium in various stages of OSMF in comparison to normal epithelium. Materials and Methods: Sample included 30 cases of OSMF of various stages and five cases of normal buccal mucosa. Measurements for epithelial thickness and cellular morphology using morphometric analysis were performed on captured digital images using image analysis software. Statistical Analysis: The ANOVA analysis of epithelial thickness and correlation of epithelial thickness with cell contour and cell roundness was done using Karl Pearson's coefficient of correlation. Results: There was a statistically significant decrease in epithelial thickness with increase in severity/stage of OSMF. It was also observed that there is a highly statistically significant correlation (P = 0.000) between the thickness of epithelium and cell morphology in varying stages of OSMF. Conclusion: The morphometric parameters and the use of quantitative histological methods in determining the squamous epithelial structures thus made it possible to evaluate that there is reduction in thickness of epithelium or “atrophy” with increasing stages of OSMF which may be due to actual change in cell morphology of the individual cells of specific compartment or all compartments in the epithelium.


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Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007