|Year : 2016 | Volume
| Issue : 3 | Page : 548
Oral adenosquamous carcinoma: Report of a rare entity with a special insight on its histochemistry
Taneeru Sravya, Guttikonda Venkateswara Rao, Manchikatla Praveen Kumar, K Sudheerkanth
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India
|Date of Submission||25-Jan-2016|
|Date of Acceptance||04-Aug-2016|
|Date of Web Publication||21-Sep-2016|
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Adenosquamous carcinoma (ASC) of the head and neck (H and N) is an aggressive variant of squamous cell carcinoma (SCC). They are described as SCC subtype with high infiltrative capacity and also presents with dual histomorphology, having both squamous and glandular cell components. ASC of the H and N region is considered as a controversial tumor, as it is similar to salivary gland mucoepidermoid carcinoma. It has been described in a variety of body sites, including uterine cervix, lung and pancreas. ASC rarely develops in the upper aerodigestive tract, particularly in the oral cavity. The affected sites in oral cavity include palate, tonsillar pillar areas and floor of the mouth. To the best of our knowledge in the literature, only 17 cases of ASC in the floor of the mouth have been reported. Hereby, we report an additional case of ASC occurring in the floor of the mouth in a 70-year-old male patient.
Keywords: Adenosquamous cell carcinoma, glandular cell component, mucoepidermoid carcinoma, squamous cell component
|How to cite this article:|
Sravya T, Rao GV, Kumar MP, Sudheerkanth K. Oral adenosquamous carcinoma: Report of a rare entity with a special insight on its histochemistry. J Oral Maxillofac Pathol 2016;20:548
|How to cite this URL:|
Sravya T, Rao GV, Kumar MP, Sudheerkanth K. Oral adenosquamous carcinoma: Report of a rare entity with a special insight on its histochemistry. J Oral Maxillofac Pathol [serial online] 2016 [cited 2021 Oct 19];20:548. Available from: https://www.jomfp.in/text.asp?2016/20/3/548/190969
| Introduction|| |
Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of epithelial cells, and the cell origin is oral keratinocyte.  Histologically, OSCC is of different types which include conventional SCC, basaloid SCC, spindle cell/sarcomatoid SCC, verrucous carcinoma, papillary SCC, adenoid/acantholytic/pseudoglandular SCC and adenosquamous carcinoma (ASC).  Each variant has its unique histological appearance. Among all these variants of OSCC, ASC is rare, aggressive malignant neoplasm in head and neck (H and N) region and has distinct squamous and glandular component as described by World Health Organization (WHO). 
According to the WHO 1995 classification of tumors of the upper respiratory tract and ear, ASC of H and N region was defined as "A malignant tumor with histological features of both adenocarcinoma and SCC."  It is also considered as a controversial tumor, as it is similar to salivary gland mucoepidermoid carcinoma (MEC), but Evans in 1984 highlighted the worse prognosis of ASC of H and N over high-grade MEC and proposed that ASC should be considered as a distinctive neoplasm. 
ASC of H and N was described by Gerughty et al., in 1968 in a series of 10 patients, where it has been shown to be extremely aggressive with 80% of patients developing metastasis.  ASC rarely develops in upper aerodigestive tract affecting the supraglottic region of larynx and oral cavity.  The most common site in oral cavity is tongue followed by palate, tonsillar pillar areas and floor of mouth.  To our knowledge in the literature, only 17 cases of ASC in the floor of the mouth have been reported. Hereby, we report an additional case of ASC occurring in the floor of the mouth in 70-year-old male patient [Table 1].
|Table 1: Review of previous cases of oral adenosquamous carcinoma reported in the floor of the mouth |
Click here to view
| Case report|| |
A 70-year-old male reported with pain in the left lower region of mouth since 2 months. The patient was a smoker and had a habit of alcohol since 50 years with unremarkable medical history. Extraoral examination revealed no facial asymmetry [Figure 1]a. Bilateral submental and submandibular lymph nodes were palpable which were tender and firm in consistency measuring approximately 1 cm × 1 cm in size. Intraoral examination revealed a small solitary ulcerated lesion with everted margins extending from lateral side of ventral portion of tongue to floor of mouth on the left side measuring approximately 7 cm × 3 cm in size [Figure 1]b. The lesion is erythematous and irregular in shape. On palpation, all the inspectory findings were confirmed and the swelling was firm in consistency. Based on these findings, a provisional diagnosis of carcinoma of the floor of the mouth was given.
Histopathological examination of incisional biopsy revealed infiltration of stratified squamous epithelium into underlying connective tissue stroma [Figure 2]a. The tumor was composed of two distinct components: Superficial squamous component and deeper glandular component [Figure 2]b. Squamous component revealed typical well differentiated SCC adjacent to dysplastic areas in the epithelium. Numerous areas of keratin pearls along with single cell keratinization were also noticed [Figure 3]a. Glandular component revealed ductal structures with varying degrees of cellular and nuclear pleomorphism [Figure 3]b. Areas of mucous cells were dispersed within the stroma. Areas of intracellular and intraluminal mucinous material and some acantholytic cells were also seen in lumen of ducts. The amorphous mucinous material within the lumen of ducts was highlighted with periodic acid-Schiff (PAS) [Figure 4]a and mucicarmine stain [Figure 4]b. Immunohistochemistry revealed strong positivity for both squamous and glandular components with respect to pancytokeratin AE1/3 and finally the case was diagnosed as adenosquamous cell carcinoma [Figure 5]. The patient was referred to cancer institute for proper treatment and follow-up.
|Figure 2: (a) Stratified squamous epithelium (indicated by arrow mark) infiltrating into underlying connective tissue stroma (indicated by an asterisk) (H&E stain, ×40). (b) Squamous component (indicated by arrow mark) and glandular component (indicated by asterisk) (H&E stain, ×100)|
Click here to view
|Figure 3: (a) Squamous component with numerous areas of keratin pearls (H&E stain, ×40) and (b) glandular component with ductal structures with smooth edges (H&E stain, ×100)|
Click here to view
|Figure 4: The adenoid component showing positivity for mucins (a) Periodic acid-Schiff stain, ×40, and (b) mucicarmine, ×40|
Click here to view
|Figure 5: Pancytokeratin AE1/3 positivity for both squamous (indicated by asterisk) and glandular component (indicated by arrow mark) of adenosquamous cell carcinoma [IHC stain, (a) ×20, (b) ×20, (c) ×40, (d) ×40]|
Click here to view
| Discussion|| |
ASC is an extremely rare tumor with <100 cases having been reported in English literature. They constitute about 0.6% and 0.1% of lung and colon cancers respectively and 1.1% of all malignant epithelial salivary gland tumors.  The histogenesis of ASC is not completely understood, but it was thought that the origin is from both glandular and surface epithelial origin.  Ellis suggested that dysplasia of the surface mucosal epithelium is an important diagnostic criteria and considered the tumor to arise synchronously from mucosal and salivary gland epithelium.  According to Fonseca et al., ASC originates from surface mucosal epithelium on the basis of surface mucosal dysplasia giving rise to invasive tumor with normal minor salivary glands in proximity.  The origin of ASC in the present case was in accordance with Ellis.
Recently, it was also proven that human papillomavirus (HPV) is an important etiological agent in H and N carcinomas particularly in the oropharynx and nasal cavity. High-risk HPV (HPV-16) has been detected in about 80% of SCC cases. The virus contributes to carcinogenesis by expressing E6 and E7 oncoproteins which bind to p53 and retinoblastoma (Rb). These are critical regulators of cell cycle and apoptosis which on disruption causes the cells to proliferate and fail to undergo apoptosis appropriately. Inactivation of Rb leads to overexpression of p16 which is a good surrogate marker of HPV. The presence of HPV, overexpression of p16 and significant expression of E6 and E7 transcripts indicates the biological and significant role of HPV in the tumor. 
In the H and N region, ASC is predominantly seen in tongue, palate, floor of the mouth and larynx with a peak incidence in the fifth decade (male:female ratio is 3:1).  Clinically, approximately 40% of patients complain of pain on the initial appointment which is mainly because of the tendency of tumor to spread by perineural invasion. Other tumor-related symptoms include odynophagia, otalgia, tongue numbness, bleeding and weight loss.  Till now, very few cases have been reported in the floor of the mouth. The present case was an additional case of ASC occurring in the floor of the mouth which was reported in 70-year-old male and was associated with pain.
As defined by WHO, ASC exhibits dual histomorphology, with both squamous and true adenocarcinoma component.  The squamous cell component usually predominates and appears in superficial areas arising from surface epithelium. The mucosal epithelium presents with severe dysplasia or carcinoma in situ. The presence of variable extent of foci of keratinization was also noticed.  The adenocarcinoma component is usually found in deeper areas which are composed of ductular structures with variable number of mucous cells. Typically, the glandular formation should consist of punched out spaces with smooth edges rather than ragged edges.  The present case also revealed similar findings, i.e. the mucosal epithelium in squamous cell component presented with severe dysplasia with focal areas of keratinization and the glandular component revealed ductular structures with smooth edges and variable mucin production.
Alos et al. suggested the following diagnostic criteria for ASC which includes: (i) The most common component is keratinizing SCC; (ii) adenocarcinoma component in the deeper portion and (iii) severe dysplasia or carcinoma in situ in the surface epithelium.  The present case was also in accordance with the above-mentioned criteria.
Histochemical examination was performed to differentiate ASC from adenoid/acantholytic SCC which revealed PAS and mucicarmine positivity for the amorphous material present within the lumen of ducts indicating true glandular formation. Immunohistochemically, the glandular component of ASC is positive for pancytokeratin, epithelial membrane antigen, CK7/8, CAM 5.2, CEA and negative for CK20 whereas squamous cell component is positive for pancytokeratin, CEA and negative for CK7/8, CK20 and CAM 5.2. , As pancytokeratin is positive for both the components, the present case was performed with the same and revealed positivity for both squamous and glandular components.
Differential diagnosis of ASC is of major importance because of its behavior [Table 2]. ASC is an aggressive tumor, with spread to cervical lymph nodes, locoregional and distant recurrence after treatment.  The treatment includes surgery alone, as well as association with radiation therapy and/or chemotherapy. A high metastatic rate about 80% and low 5 years survival rate which is about 20-25% was expected. 
| Conclusion|| |
ASC is a rare malignant neoplasm which is aggressive and associated with poor outcome when compared to conventional SCC and also presents with high rate of lymph node metastasis. ASC should be ruled out from MEC, basaloid SCC and adenoid SCC. The precise histopathological diagnosis helps the clinician to plan the accurate treatment.
Declaration of patient consent
The authors certify that they have obtained all appropriate patient consent forms. In the form the patient(s) has/have given his/her/their consent for his/her/their images and other clinical information to be reported in the journal. The patients understand that their names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Scully C, Bagan J. Oral squamous cell carcinoma overview. Oral Oncol 2009;45:301-8.
Pathak J, Swain N, Patel S, Poonja L. Histopathological variants of oral squamous cell carcinoma-institutional case reports. J Oral Maxillofac Pathol 2014;18:143-5.
Abdelsayed RA, Sangueza OP, Newhouse RF, Singh BS. Adenosquamous carcinoma: A case report with immunohistochemical evaluation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:173-7.
Shanmugaratnam K, Sobin LH. Adenosquamous carcinoma. In: Histological Typing of Tumors of the Upper Respiratory Tract and Ear. World Health Organization, International Histological Classification of Tumors. Berlin: Springer-Verlag; 1991. p. 38.
Alos L, Castillo M, Nadal A, Caballero M, Mallofre C, Palacin A, et al.
Adenosquamous carcinoma of the head and neck: Criteria for diagnosis in a study of 12 cases. Histopathology 2004;44:570-9.
Gerughty RM, Hennigar GR, Brown FM. Adenosquamous carcinoma of the nasal, oral and laryngeal cavities. A clinicopathologic survey of ten cases. Cancer 1968;22:1140-55.
Carvalho AT, Cazal C, Castro JF, Leao JC, Porter SR. Adenosquamous carcinoma of the floor of the mouth mimicking an oral erythroleukoplakia: Case report and review of literature. Appl Cancer Res 2009;29:135-9.
Siar CH, Ng KH. Adenosquamous carcinoma of the floor of the mouth and lower alveolus: A radiation-induced lesion? Oral Surg Oral Med Oral Pathol 1987;63:216-20.
Yoshimura Y, Mishima K, Obara S, Yoshimura H, Maruyama R. Clinical characteristics of oral adenosquamous carcinoma: Report of a case and an analysis of the reported Japanese cases. Oral Oncol 2003;39:309-15.
Schick U, Pusztaszeri M, Betz M, Ghadjar P, Demiroz C, Kaanders JH, et al.
Adenosquamous carcinoma of the head and neck: Report of 20 cases and review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol 2013;116:313-20.
Shyamala K, Kanjikar S, Girish HC, Murgod S. Adenosquamous carcinoma: Histogenetic and molecular insight with a report of a case. J Med Radiol Pathol Surg 2015;1:37-9.
Ellis GL, Auclair PL, Gnepp DR, Goode RK. Adenosquamous cell carcinoma. In: Ellis GL, Auclair PL, Gnepp DR, editors. Surgical Pathology of the Salivary Glands. Philadelphia: WB, Saunders; 1991. p. 455-9.
Fonseca FP, Ramos LM, Vargas PA, de Almeida OP, Lopes MA, Santos-Silva AR. Oral adenosquamous carcinoma: Evidence that it arises from the surface mucosal epithelium. Histopathology 2012;61:321-3.
Masand RP, El-Mofty SK, Ma XJ, Luo Y, Flanagan JJ, Lewis JS Jr. Adenosquamous carcinoma of the head and neck: Relationship to human papillomavirus and review of the literature. Head Neck Pathol 2011;5:108-16.
Kusafuka K, Miki T, Nakajima T. Adenosquamous carcinoma of the parotid gland. Histopathology 2013;63:593-5.
Sheahan P, Fitzgibbon J, Lee G, O'Leary G. Adenosquamous carcinoma of the tongue in a 22-year-old female: Report of a case with immunohistochemistry. Eur Arch Otorhinolaryngol 2003;260:509-12.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
[Table 1], [Table 2]