Year : 2017 | Volume
: 21 | Issue : 3 | Page : 367--374
Kerosene: Contributing agent to xylene as a clearing agent in tissue processing
Amisha Ashokkumar Shah1, Dinraj Kulkarni1, Yashwant Ingale1, Ajit V Koshy1, Sanjay Bhagalia2, Nikhil Bomble3
1 Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Sciences and Research Centre, Pune, India
2 Department of Oral Pathology and Microbiology, Rural Dental College, Pravara Institute of Medical Sciences, Loni, India
3 Late Shri Yashwantrao Chavan Memorial Medical and Rural Development Foundation Dental College and Hospital, Ahmednagar, Maharashtra, India
Background: Research methodology in oral and maxillofacial pathology has illimitable potential. The tissue processing involves many steps of which one of the most important step is “Clearing,” which is a process of replacing dehydrant with a substance which is miscible with embedding medium or paraffin wax. Xylene is one of the common clearing agents used in laboratory, but it is also hazardous. The main aim of this study is to substitute conventionally used xylene by a mixture of kerosene and xylene in clearing steps without altering the morphology and staining characteristics of tissue sections. This will also minimize the toxic effects and tend to be more economical.
Materials and Methods: One hundred and twenty bits of tissue samples were collected, each randomly separated into 4 groups (A, B, C and D) and kept for routine tissue processing till the step of clearing; during the step of clearing instead of conventional xylene, we used mixture of xylene and kerosene in 4 ratios ([A-K:X – 50:50]; [B-K:X – 70:30]; [C – Ab. Kerosene]; [D – Ab. Xylene – as control]) and observed for the light microscopic study adopting H and E staining, IHC (D2-40), Special stains (periodic acid–Schiff and congo red) procedure. The result was subjected to statistical analysis by using Fisher's exact test.
Results: The results obtained from the present study were compared with control group, i.e., D and it was observed that Groups A and B were absolutely cleared without altering the morphology of tissue and cellular details; optimum embedding characteristics and better staining characteristics were also noted, whereas Group C presents poor staining characteristics with reduced cellular details. Embedded tissues in Group C presented with rough, irregular surface and also appeared shrunken.
Conclusion: Combined mixture of xylene and kerosene as a clearing agent in different ratio, i.e., Group A (K:X – 50:50) and B (K:X – 70:30) can be used without posing any health risk or compromising the cellular integrity.
Dr. Amisha Ashokkumar Shah
E/502, Nancy Lake Homes Opp. Bharati Vidyapeeth, Katraj, Pune - 411 046, Maharashtra
|How to cite this article:|
Shah AA, Kulkarni D, Ingale Y, Koshy AV, Bhagalia S, Bomble N. Kerosene: Contributing agent to xylene as a clearing agent in tissue processing.J Oral Maxillofac Pathol 2017;21:367-374
|How to cite this URL:|
Shah AA, Kulkarni D, Ingale Y, Koshy AV, Bhagalia S, Bomble N. Kerosene: Contributing agent to xylene as a clearing agent in tissue processing. J Oral Maxillofac Pathol [serial online] 2017 [cited 2020 May 29 ];21:367-374
Available from: http://www.jomfp.in/article.asp?issn=0973-029X;year=2017;volume=21;issue=3;spage=367;epage=374;aulast=Shah;type=0