Journal of Oral and Maxillofacial Pathology

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 20  |  Issue : 3  |  Page : 342--347

To analyze the concomitant expression of human papillomavirus-16 in the pathogenetic model of p53-dependant pathway in oral squamous cell carcinoma


Ajit Singh Rathore, Nikita Gulati, Devi Charan Shetty, Anshi Jain 
 Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Ghaziabad, Uttar Pradesh, India

Correspondence Address:
Anshi Jain
Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental Collge, Murad Nagar, Ghaziabad - 201 206, Uttar Pradesh
India

Background: Oral squamous cell carcinoma (OSCC) accounts for 90% of all primary oral malignancies. Association between human papillomavirus (HPV) as a risk factor of cervical cancer is well known; there is a need to widen the knowledge for its role in oral cancer development. The viral E6 protein of HPV binds to p53 making it nonfunctional. Aims and Objective: To study mutated/wild type p53 expression using immunohistochemistry and detect HPV-16 presence using polymerase chain reaction (PCR), in OSCC and correlating their expression . Materials and Methods: Immunohistochemical staining for p53 molecule in 24 sections of OSCC followed by DNA extraction of the cases using qiagen extraction kit and subsequent HPV-16 detection using PCR technique. Statistical Analysis : The data were analysed using SPSS software version 19. Results: Out of 24 cases of OSCC, twenty cases were positive for P 53 expression and four cases were negative for P 53 expression. Out of the four negative cases, one case was detected positive for HPV-16. Conclusion: HPV infection along with p53 expression helps in understanding its exact pathogenesis which further helps in expanding our spectrum of therapeutic modalities


How to cite this article:
Rathore AS, Gulati N, Shetty DC, Jain A. To analyze the concomitant expression of human papillomavirus-16 in the pathogenetic model of p53-dependant pathway in oral squamous cell carcinoma.J Oral Maxillofac Pathol 2016;20:342-347


How to cite this URL:
Rathore AS, Gulati N, Shetty DC, Jain A. To analyze the concomitant expression of human papillomavirus-16 in the pathogenetic model of p53-dependant pathway in oral squamous cell carcinoma. J Oral Maxillofac Pathol [serial online] 2016 [cited 2017 Apr 28 ];20:342-347
Available from: http://www.jomfp.in/article.asp?issn=0973-029X;year=2016;volume=20;issue=3;spage=342;epage=347;aulast=Rathore;type=0