Year : 2009 | Volume
: 13 | Issue : 1 | Page : 2--8
Candida and calcofluor white: Study in precancer and cancer
Rashmi Santosh Kumar1, SM Ganvir2, VK Hazarey2
1 Department of Oral and Maxillofacial Pathology and Microbiology, Kamineni Institute of Dental Sciences, Narketpalli - 508254, Andhra Pradesh, India
2 Department of Oral and Maxillofacial Pathology and Microbiology, GDC and H Nagpur, Maharashtra, India
Background: The interest in oral candidosis has waxed and waned from the period of Hippocrates. The acquired immune deficiency syndrome (AIDS) epidemic has certainly bolstered these figures on oral candidosis, with diabetes and oral cancer being no exception. A need for rapid detection of Candida is made possible by the use of Calcofluor - White (CFW) stain when examined under a fluorescence microscope. The present study was aimed at assessing the efficacy of CFW is compared to Gram stain and periodic acid Schiff (PAS) in detection of Candida in oral precancer and cancer.
Materials and Methods: The study group consisted of patients with precancer (n=45), cancer (n=45), and control group (n=45). Presence of Candida was confirmed by culture inoculation along with a germ tube and carbohydrate fermentation test. The cytopathological smears were analyzed by papanicolaou - CFW and Gram staining, whereas, tissue sections were stained by PAS and CFW staining.
Results: Candida albicans was the predominant species identified. A highly significant association of Candida was seen more often in cancer than in precancer. Both in cytology and histopathology Candida detection by CFW was higher. In precancer it was 48.88% in smears and 40% in tissue sections, whereas, in cancer 60% in smears and 55.55% in histopathology.
Conclusion: Among the various diagnostic tools used in the present study, the use of CFW is seen to be a simple, effective, rapid, and reliable method, both in cytopathology and histopathology.
Rashmi Santosh Kumar
Department of Oral and Maxillofacial Pathology and Microbiology, Kamineni Institute of Dental Sciences, Narketpalli - 508 254, Andhra Pradesh
|How to cite this article:|
Kumar RS, Ganvir S M, Hazarey V K. Candida and calcofluor white: Study in precancer and cancer.J Oral Maxillofac Pathol 2009;13:2-8
|How to cite this URL:|
Kumar RS, Ganvir S M, Hazarey V K. Candida and calcofluor white: Study in precancer and cancer. J Oral Maxillofac Pathol [serial online] 2009 [cited 2020 Sep 26 ];13:2-8
Available from: http://www.jomfp.in/article.asp?issn=0973-029X;year=2009;volume=13;issue=1;spage=2;epage=8;aulast=Kumar;type=0