Year : 2007 | Volume
: 11 | Issue : 1 | Page : 15--17
Comparison of three different methods of tissue processing
Pritam Panja, G Sriram, TR Saraswathi, B Sivapathasundharam
Department of Oral and Maxillofacial Pathology, Meenakshi Ammal Dental College, Chennai, India
Biopsied tissue is cut into thin slices and stained suitably for microscopical examination. Enabling the tissue for sectioning by paraffin embedding is known as tissue processing. The three most commonly employed means of tissue processing are routine manual method, rapid manual method and the microwave method.
In this study, sections obtained from the same site of the same tissue were processed by these three methods and stained by hematoxylin and eosin. These sections were then microscopically evaluated by various parameters to compare the three methods.
The results that were obtained, after subjecting to statistical analysis, showed no significant differences between the three different processes in terms of quality of staining, clarity of nucleo-cytoplasmic differentiation in various cells and the presence of artifacts. Tissue shrinkage was less in microwave-processed tissue as compared to the other methods. Microwave tissue processing was also found to be more cost-effective than other methods.
Department of Oral and Maxillofacial Pathology, Meenakshi Ammal Dental College, Chennai
|How to cite this article:|
Panja P, Sriram G, Saraswathi T R, Sivapathasundharam B. Comparison of three different methods of tissue processing.J Oral Maxillofac Pathol 2007;11:15-17
|How to cite this URL:|
Panja P, Sriram G, Saraswathi T R, Sivapathasundharam B. Comparison of three different methods of tissue processing. J Oral Maxillofac Pathol [serial online] 2007 [cited 2019 Dec 9 ];11:15-17
Available from: http://www.jomfp.in/article.asp?issn=0973-029X;year=2007;volume=11;issue=1;spage=15;epage=17;aulast=Panja;type=0