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An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
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Year : 2018  |  Volume : 22  |  Issue : 3  |  Page : 448
Demystifying the nature of hard tissues in odontogenic tumors using Modified Gallego's stain: A preliminary study


Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India

Correspondence Address:
Syeda Neelam Afroze
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jomfp.JOMFP_33_18

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Introduction: Histological stains are dyes that bind to a variety of tissues. Modified Gallego's (MG) stain is a modification of Lille's stain that can be used as a differential stain for identification of hard tissues in oral pathological lesions. Objectives: The objective of this study was to identify the presence of hard tissues such as enamel, dentin and cementum in normal extracted teeth and odontogenic tumors using MG stain and to compare the efficacy of MG stain with hematoxylin and eosin (H&E) stain. Methods: A total of fifty samples, twenty decalcified sections of teeth and thirty cases of odontogenic tumors, were included in the present study. Two sections were cut from the above cases and stained with H&E stain and MG stain, respectively, and assessed for the nature of hard tissue. Results: In H&E staining, enamel, dentine, cementum and bone stained pink. Whereas, in MG stain, enamel stained pink, dentin and bone stained green, while cementum stained red. The shade of color differs with the degree of mineralization of the hard tissues in MG stain. Conclusion: MG stain can be used as a differential stain for different hard-tissue structures when compared to routine H and E staining.


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Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
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