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An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
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Year : 2018  |  Volume : 22  |  Issue : 3  |  Page : 443
Rapid tissue processing technique: A novel method using methyl salicylate


1 Department of Oral Pathology and Microbiology, Rajarajeswari Dental College and Hospital, Bengaluru, Karnataka, India
2 Department of Oral Pathology and Microbiology, DAPMRV Dental College and Hospital, Bengaluru, Karnataka, India

Correspondence Address:
Shyamala Karnam
Department of Oral Pathology and Microbiology, Rajarajeswari Dental College and Hospital, No. 14, Ramohalli Cross, Kumbalgodu, Mysore Road, Bengaluru - 560 060, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jomfp.JOMFP_228_17

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Introduction: Tissue processing involves transition of the biopsy tissue in graded concentration of various chemicals to make the tissue amiable for sectioning. The entire process takes 2–3 working days before a microscopic slide is ready for diagnosis. In order to shorten the turnaround time, rapid tissue processing method using methyl salicylate was developed. Aim: The aim of this study is to develop a rapid tissue processing technique using methyl salicylate as a clearing agent and to compare it with routine tissue processing technique. Materials and Methods: A total of 70 tissue specimens were cut into two equal halves. One each was processed by routine processing technique (RoPT) and rapid processing technique (RaPT). Tissue specimens were measured before and after processing. Quality of staining and cellular-level shrinkage were observed and scored for specimens. Statistical analysis using Welch's unequal variances t-test was performed. Costs of chemicals in both the techniques were compared to see the cost-effectiveness of RaPT. Results and Conclusion: Outcomes of both the processing techniques were comparable with statistically not significant P values for all the parameters. Hence, the results of RaPT technique are satisfactory, and the use of this technique may prove beneficial to pathology laboratories.


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Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007