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| ORIGINAL ARTICLE |
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| Year : 2018 |
Volume
: 22 | Issue : 2 | Page
: 283 |
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Comparison of efficacy of local anesthetic solution, distilled water and normal saline as emergency fixatives
Sowmya Kasetty1, Dhara Dwivedi2, T Raju Ragavendra1, Shreenivas Kallianpur2, Sandeep Gupta3, Nitin Prabhakar4
1 Department of Oral Basic and Clinical Sciences, College of Dentistry, Buraydah, KSA
2 Department of Oral Pathology, People's College of Dental Science and Research Centre, Bhopal, Madhya Pradesh, India
3 Department of Oral Pathology and Microbiology, RKDF, Bhopal, Madhya Pradesh, India
4 Department of Oral Maxillofacial Surgery, Ayder Referral Hospital - College of Health Sciences, Mekelle University, Ethiopia
Correspondence Address:
Sowmya Kasetty
Department of Oral Basic and Clinical Sciences, College of Dentistry, Buraidah
KSA
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jomfp.JOMFP_189_16
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Context: Adequate tissue fixation is fundamental to good quality histological sections. Owing to undesirable effects of 10% buffered formalin, its availability in clinics is questionable. Thus, the present study was conducted with a novel approach to fixation, together with the scope of finding fixative properties of more commonly used reagents available at the clinics.
Aims: The present study was aimed to compare the efficacy of local anesthetic solution, normal saline (NS) and distilled water (DW) with that of 10% neutral-buffered formalin.
Settings and Design: It is a single-blinded study where histological assessment of fixation was done to assert if the tissues procured were sufficient or insufficient for the clinical diagnosis with/without any problems.
Subjects and Methods: Forty soft-tissue specimens obtained from 2 goat tongue were used. Tissues each were directly immersed in local anesthesia, DW, NS solution and formalin for 12 and 24 h each and labeled as Group I and Group II, respectively. The sections were evaluated for staining quality and were subjected to statistical analysis.
Statistical Analysis Used: Kruskal–Wallis test was employed to assess the differences in histological quality scores. Comparison between the tissues of the two groups was estimated with Mann–Whitney U-test. Kappa Statistic was used to measure the interobserver variation.
Results: There was a significant difference (P ≤ 0.05) in the efficacy of all the three emergency fixatives.
Conclusions: On the basis of the results obtained, local anesthetic solution can be used as an emergency fixative.
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