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An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists

ORIGINAL ARTICLE Table of Contents   
Year : 2018  |  Volume : 22  |  Issue : 2  |  Page : 283
Comparison of efficacy of local anesthetic solution, distilled water and normal saline as emergency fixatives

1 Department of Oral Basic and Clinical Sciences, College of Dentistry, Buraydah, KSA
2 Department of Oral Pathology, People's College of Dental Science and Research Centre, Bhopal, Madhya Pradesh, India
3 Department of Oral Pathology and Microbiology, RKDF, Bhopal, Madhya Pradesh, India
4 Department of Oral Maxillofacial Surgery, Ayder Referral Hospital - College of Health Sciences, Mekelle University, Ethiopia

Correspondence Address:
Sowmya Kasetty
Department of Oral Basic and Clinical Sciences, College of Dentistry, Buraidah
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jomfp.JOMFP_189_16

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Context: Adequate tissue fixation is fundamental to good quality histological sections. Owing to undesirable effects of 10% buffered formalin, its availability in clinics is questionable. Thus, the present study was conducted with a novel approach to fixation, together with the scope of finding fixative properties of more commonly used reagents available at the clinics. Aims: The present study was aimed to compare the efficacy of local anesthetic solution, normal saline (NS) and distilled water (DW) with that of 10% neutral-buffered formalin. Settings and Design: It is a single-blinded study where histological assessment of fixation was done to assert if the tissues procured were sufficient or insufficient for the clinical diagnosis with/without any problems. Subjects and Methods: Forty soft-tissue specimens obtained from 2 goat tongue were used. Tissues each were directly immersed in local anesthesia, DW, NS solution and formalin for 12 and 24 h each and labeled as Group I and Group II, respectively. The sections were evaluated for staining quality and were subjected to statistical analysis. Statistical Analysis Used: Kruskal–Wallis test was employed to assess the differences in histological quality scores. Comparison between the tissues of the two groups was estimated with Mann–Whitney U-test. Kappa Statistic was used to measure the interobserver variation. Results: There was a significant difference (P ≤ 0.05) in the efficacy of all the three emergency fixatives. Conclusions: On the basis of the results obtained, local anesthetic solution can be used as an emergency fixative.

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Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
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