Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contact Us Login 
An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
ORIGINAL ARTICLE Table of Contents   
Year : 2017  |  Volume : 21  |  Issue : 3  |  Page : 367-374
Kerosene: Contributing agent to xylene as a clearing agent in tissue processing


1 Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Sciences and Research Centre, Pune, India
2 Department of Oral Pathology and Microbiology, Rural Dental College, Pravara Institute of Medical Sciences, Loni, India
3 Late Shri Yashwantrao Chavan Memorial Medical and Rural Development Foundation Dental College and Hospital, Ahmednagar, Maharashtra, India

Correspondence Address:
Dr. Amisha Ashokkumar Shah
E/502, Nancy Lake Homes Opp. Bharati Vidyapeeth, Katraj, Pune - 411 046, Maharashtra
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jomfp.JOMFP_14_15

Rights and Permissions

Background: Research methodology in oral and maxillofacial pathology has illimitable potential. The tissue processing involves many steps of which one of the most important step is “Clearing,” which is a process of replacing dehydrant with a substance which is miscible with embedding medium or paraffin wax. Xylene is one of the common clearing agents used in laboratory, but it is also hazardous. The main aim of this study is to substitute conventionally used xylene by a mixture of kerosene and xylene in clearing steps without altering the morphology and staining characteristics of tissue sections. This will also minimize the toxic effects and tend to be more economical. Materials and Methods: One hundred and twenty bits of tissue samples were collected, each randomly separated into 4 groups (A, B, C and D) and kept for routine tissue processing till the step of clearing; during the step of clearing instead of conventional xylene, we used mixture of xylene and kerosene in 4 ratios ([A-K:X – 50:50]; [B-K:X – 70:30]; [C – Ab. Kerosene]; [D – Ab. Xylene – as control]) and observed for the light microscopic study adopting H and E staining, IHC (D2-40), Special stains (periodic acid–Schiff and congo red) procedure. The result was subjected to statistical analysis by using Fisher's exact test. Results: The results obtained from the present study were compared with control group, i.e., D and it was observed that Groups A and B were absolutely cleared without altering the morphology of tissue and cellular details; optimum embedding characteristics and better staining characteristics were also noted, whereas Group C presents poor staining characteristics with reduced cellular details. Embedded tissues in Group C presented with rough, irregular surface and also appeared shrunken. Conclusion: Combined mixture of xylene and kerosene as a clearing agent in different ratio, i.e., Group A (K:X – 50:50) and B (K:X – 70:30) can be used without posing any health risk or compromising the cellular integrity.


[FULL TEXT] [PDF]*
Print this article  Email this article
    

  Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
   Citation Manager
  Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed724    
    Printed6    
    Emailed0    
    PDF Downloaded101    
    Comments [Add]    

Recommend this journal

 

Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007