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An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
ORIGINAL ARTICLE Table of Contents   
Year : 2014  |  Volume : 18  |  Issue : 1  |  Page : 14-18
Vinegar as a disinfectant of extracted human teeth for dental educational use


1 Department of Oral Pathology and Microbiology, People's College of Dental Sciences and Research Centre, People's University, Bhopal, Madhya Pradesh, India
2 Department of Public Health Dentistry, People's College of Dental Sciences and Research Centre, People's University, Bhopal, Madhya Pradesh, India

Correspondence Address:
D Smitha
Department of Oral Pathology and Microbiology, People's College of Dental Sciences and Research Centre, People's University, Bhanpur, Bhopal 462 037, Madhya Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-029X.131883

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Context: Extracted human teeth are routinely used in dentistry to learn technical and preclinical skills. Centers for Disease Control and Prevention (CDC) has adopted guidelines for infection control of extracted teeth used for research and teaching, requiring that teeth be sterilized before use. Many of the proposed disinfection methods starting from use of formalin, sodium hypochlorite and to autoclaving have their own drawbacks and may not be practical. Aim: To assess the effectiveness of vinegar for disinfection/sterilization of extracted human teeth. Materials and Methods: A total of 80 extracted non-carious human teeth were kept in seven disinfectant media-10% formalin, 3% hydrogen peroxide, 5.25% sodium hypochlorite, 70% alcohol, vinegar, neem extract and normal saline. Ten samples were placed in each disinfectant individually for a period of 7 days, at room temperature. In all, 10 teeth were treated with microwave irradiation at 650 W for 3 min. Later, teeth from each group were placed individually in separate test tubes containing 10 ml of tryptic soy broth at 37°C for 48 h to observe the evidence of growth of microorganisms. Semiquantitative analysis of all the samples was done in Clade agar at 37°C for 48 h. Statistical Analysis Used: The number of teeth disinfected in each group was compared using Chi square test. Results: 10% formalin, 3% hydrogen peroxide and vinegar were totally effective. The result was statistically significant with a Chi square value of 61.414 and P < 0.001. Conclusions: Vinegar can be used as an effective disinfectant medium for extracted human teeth.


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Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007