Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contact Us Login 
An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
ORIGINAL ARTICLE Table of Contents   
Year : 2011  |  Volume : 15  |  Issue : 3  |  Page : 272-277
α4β1 integrin-dependent cell sorting dictates T-cell recruitment in oral submucous fibrosis


1 Departments of Oral Medicine and Diagnostic Science, College of Dentistry, King Saud University, Riyadh, Saudi Arabia
2 Department of Oral Pathology, Government Dental College, Trivandrum, India
3 Departments of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh, Saudi Arabia
4 Departments of Periodontics and Community Dentistry, and Dental Implant and Osseointegration Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia

Correspondence Address:
R Rajendran
College of Dentistry, King Saud University, P.O. Box: 60169, Riyadh 11545
Saudi Arabia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Read associated Retraction Notice: Retraction Notice with this article

DOI: 10.4103/0973-029X.86678

Rights and Permissions

Aim: The biological mechanism(s) that guide the immunological effectors of lymphocytes to sites of inflammatory response, a feature consistently seen in oral submucous fibrosis (OSF) was evaluated. It is envisaged that endothelial/lymphocyte adhesion cascades involving VCAM-1/α4β1 integrins control the migration of lymphocytes across the vascular endothelium resulting in their homing in these locales. Materials and Methods: The study group comprised 28 OSF cases (M:F = 12:16, age range 18-65 years; mean 55.4 ± 8.5 SD) divided into early (n=17) and advanced (n=11) disease groups. Biopsy specimens of normal buccal mucosa (site compatible) were obtained from 10 healthy volunteers (age and sex matched) who served as control. All the samples were fixed in 10% neutral-buffered formalin and embedded in paraffin. Immunolocalization of b1 subunit associated with α4 integrin was performed by a mouse heterodimer (clone 4B7R, Ig G, R & D Systems Inc., dilution 1:100) using a peroxidase labeled streptavidin-biotin technique. The immunocompetent cell density was expressed as the number of positive cells per mm 2 . The Mann-Whitney U-test and Fischer exact test were used to evaluate differences. P<0.05 was considered to be significant. Results: The median percentage of "T" lymphocytes with positive integrin α4β1 expression was 77.7 (an interquartile range of 73.3-83.4) for the test cases and for the controls, it was 28.2 (IQR 24.0-38.3). This difference was significant at 0.001 level. For the endothelial cells the positive expression was 82.8 (IQR 77-90.6) and 22.3 (IQR 18.3-29.2) respectively (P<0.001). When the intensity of integrin expression was considered 26/28 cases (96%) and 2/10 (20%) of controls showed intense expression of integrins a4b1 on T lymphocytes (P<0.001). Similarly, 27/28 cases (92.9%) and 2/10 (20%) of controls showed intense expression on endothelial cells (P<0.001). T lymphocyte-endothelial cell interactions were assessed by evaluating the overexpression of integrins on both the endothelial cells and lymphocytes together. The interaction was positive in 15/17 and 11/11 early and advanced OSF cases respectively (P=0.51). Conclusion: Following leukocyte activation, the interaction between leukocyte integrin heterodimers and endothelial superfamily adhesion ligands results in a firm adherence of leukocytes to endothelium, leading to leukocyte migration and homing to sites of mucosal inflammation consistently seen in OSF.


[FULL TEXT] [PDF Not available]*
Print this article  Email this article
    

  Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
   Citation Manager
  Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1533    
    Printed85    
    Emailed0    
    PDF Downloaded106    
    Comments [Add]    

Recommend this journal

 

Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007