Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contact Us Login 
An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists

ORIGINAL ARTICLE Table of Contents   
Year : 2011  |  Volume : 15  |  Issue : 1  |  Page : 26-33
Morphometry of the basal cell layer of oral leukoplakia and oral squamous cell carcinoma using computer-aided image analysis

1 Department of Oral and Maxillofacial Pathology, V. S. Dental College and Hospital, K. R. Road, VV Puram, Bangalore, India
2 AECS Maruti College of Dental Sciences and Research Centre, Bannerghatta Road, Bangalore, India
3 Rajarajeshwari Dental College, Kumbalgodu, Ramohalli Cross, Bangalore, India

Correspondence Address:
T Smitha
Department of Oral and Maxillofacial Pathology, V. S. Dental College and Hospital, K. R. Road, VV Puram, Bangalore
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-029X.80034

Rights and Permissions

Objectives: To study and compare the changes in nuclear and cellular size, shape and nuclear-cytoplasmic ratio of the cells in the basal layer of oral leukoplakia and well-differentiated oral squamous cell carcinoma (SCC) with normal buccal mucosa, using computer-aided image analysis in tissue sections. Study design: This was a retrospective study conducted on tissue sections on a total number of 70 cases to determine the various morphometric parameters. The data collected in this study were analyzed statistically by computing descriptive statistics, viz., percentage, mean, standard deviation, standard error of mean, 95% confidence interval for mean. The difference in the control and study groups for various diagnostic variables was compared by means of analysis of variance (ANOVA), Student's t-test for independent samples, wherever applicable. Mann-Whitney U-test and Kruskal-Wallis test were used where the data were found to be asymmetrical and the standard deviations were also different. The results were considered statistically significant whenever P ≤ 0.05. Results: Our results were significant for the morphometric parameter, size. The values of nuclear perimeter and area, cellular perimeter and area increased gradually from the normal buccal mucosa to leukoplakia, reaching the highest value in SCC. There was statistically significant difference in the nuclear and cellular areas to differentiate between leukoplakia and squamous cell carcinoma. Two variables which were used to study the shape, "form perimeter (PE)" and "contour index (CI)", showed significant difference between normal buccal mucosa and leukoplakia and between normal buccal mucosa and SCC. The morphometric parameter, nuclear-cytoplasmic ratio, in our results showed an increase in leukoplakia and SCC compared to normal buccal mucosa, but the difference was not significant between leukoplakia and SCC. Conclusion: The morphometric parameter, size, was useful to differentiate between normal, potentially malignant leukoplakia and SCC.

Print this article  Email this article

  Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
   Citation Manager
  Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded720    
    Comments [Add]    
    Cited by others 4    

Recommend this journal


Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007